What Is Pullulanase

Just fill in the form below, click submit, you will get the price list, and we will contact you within one working day. Please also feel free to contact us via email or phone. (* is required).

  • Pullulanase - an overview | ScienceDirect Topics

    2021-1-28 · 普鲁兰酶 ( pullulanase) 是一类淀粉 脱支酶 ,因其能专一性 水解 普鲁兰糖 ( pullulan, 麦芽三糖 以α-1,6 糖苷键 连接起来的 聚合物) 而得名,属 淀粉酶 类,能够 专一性 切开 支链淀粉 分支点中的α-1,6糖苷键,切下整个分支结构,形成 直链淀粉 [2]。.

    Get Price
  • Pullulanase - an overview | ScienceDirect Topics

    Bacterial pullulanases are usually thermostable, as shown by those from the genera Clostridium, Thermoanaerobacter, and Thermobacteroides.. 1204 In addition to α-(1 6) linkages, they cleave α-(1 4)-linkages as with, for instance, the pullulanase from Bacillus sp. 3183. 1205 Pullulanase from B. subtilis 1169,1206,1207 is thermostable, but that from B. acidopullulyticus (M w 102 kDa) …

    Get Price
  • Pullulanase - Creative Enzymes

    Pullulanase is a single polypeptide enzyme of ∼ 100 kDa in mass. Grains from a rice pullulanase mutant contain starch with a reduced proportion of short chains (DP 13 and below) and a slightly lower gelatinization temperature (lower by 1–3 °C).

    Get Price
  • Pullulanase: role in starch hydrolysis and potential ...

    Pullulanase, an important debranching enzyme, has been widely utilised to hydrolyse the α-1,6 glucosidic linkages in starch, amylopectin, pullulan, and related oligosaccharides, which enables a complete and efficient conversion of the branched polysaccharides into small fermentable sugars during saccharification process.

    Get Price
  • Pullulanase | SpringerLink

    2016-7-16 · Pullulanase. Pulmonary Adenoma. Pulmonary Emphysema. Pulmonary Hypertension. Pulmonary Surfectant Proteins. Pulmonary Stenosis. Pulse-Chase Analysis. Pulsed Field Gel Electrophoresis. Puma Cat (Felis concolor, Puma concolor) PUMA (p53 upregulated modulator of apoptosis) PUMA2. Pump.

    Get Price
  • Pullulanase - WikiMili, The Free Encyclopedia

    2019-9-30 · Pullulanase (EC 3.2.1.41, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, alpha-dextrin endo-1,6-alpha-glucosidase, R-enzyme, pullulan alpha-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. [1] [2] [3] It is produced as an extracellular, cell surface-anchored lipoprotein by …

    Get Price
  • Pullulanase – Wikipedia

    2021-5-25 · Die Pullulanase (auch Pullulan-6-Glucanohydrolase) ist ein bakterielles Enzym, das unter anderem von Klebsiella synthetisiert und exprimiert wird und den extrazellulären Abbau des pflanzlichen Polysaccharids Pullulan erlaubt. Solche Enzyme nennt man auch Exoenzyme.Die Monomere werden anschließend von den Bakterien aufgenommen.. Weblinks. Infos über Wirkung und Verwendung von …

    Get Price
  • Pullulanase — Wikipédia

    2021-5-12 · La pullulanase est une glycoside hydrolase qui catalyse l' hydrolyse des liaisons osidiques α-D- (16) du pullulane, de l' amylopectine et du glycogène, ainsi que celles des dextrines limites α et β de ces deux derniers.

    Get Price
  • Pullulanasi - Wikipedia

    2021-5-27 · Da Wikipedia, l'enciclopedia libera. La pullulanasi è un enzima appartenente alla classe delle idrolasi, che catalizza l' idrolisi dei legami (16)-α-D-glucosidici del pullulano, dell' amilopectina e …

    Get Price
  • Pullulanase - Creative Enzymes

    Pullulanase is a class of enzymes that can catalyze the hydrolysis of a-1,6-glycosidic bonds in starch and other polysaccharide compounds to form amylose. According to the differences in protein structure and the classification basis of the Glycoside Hydrolase family (GH), in general, pullulanase can be classified into two different glycoside ...

    Get Price
  • Pullulanase - Substance Information - ECHA

    2019-8-16 · 英文名: pullulanase 其它名称: 茁酶多糖酶 酶活力定义: 在60℃、pH4.5条件下,1min水解普鲁兰多糖(以葡萄糖计)所需酶量为一个酶活力单位。主要用途: 提高麦汁可发酵度和改变麦汁糖化的组分,长应用于干爽型啤酒和低热能啤酒的酿造。

    Get Price
  • Pullulanase M1 Klebsiella planticola Enzyme | Megazyme

    Active enzyme protein of pullulanase (EC no. 232-983-9, CAS no. 9075-68-7, EC name: Pullulanase, Enzyme Class No.: 3.2.1.41) Registration dossier Pullulanase

    Get Price
  • pulA - Pullulanase, type I - Lactobacillus crispatus ...

    One Unit of pullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40 o C.

    Get Price
  • Pullulanase:RoleinStarchHydrolysisand ...

    2016-6-8 · cd10315, CBM41_pullulanase, 1 hit: Gene3D i: 2.60.40.10, 1 hit: InterPro i: View protein in InterPro IPR013784, Carb-bd-like_fold IPR005323, CBM41_pullulanase IPR006047, Glyco_hydro_13_cat_dom IPR004193, Glyco_hydro_13_N IPR017853, Glycoside_hydrolase_SF IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR011840 ...

    Get Price
  • pul - Pullulanase - Thermus thermophilus - pul gene

    2012-3-26 · Pullulanase. Pullulanase with EC 3.2.1.41 or also known as α-dextrin 6-glucanohydrolase, pullulan 6-glucanohydrolase, limit dextrinase, and amylopectin 6-glucanohydrolase is derived from various microorganisms such as Bacillus aci-dopullulyticus,Klebsiellaplanticola[21],Bacillusderamificans [22], thermophilic Bacillus sp. AN-7 [23], Bacillus ...

    Get Price
  • Pullulanase Market Share, Growth Rate, Forecast to

    2004-7-5 · « hide 10 20 30 40 50 mlhisrtfaa yldemdqivv lapkslgfdg mapftlvaps geeiplsvqh 60 70 80 90 100 vedvgetvky vcrfasafef gatywvrscr geetdvqiga vvrtpafddr 110 120 130 140 150 ffydgplgae ylkeqtvfrv waptatavsv klvhphldei rcvplvrger 160 170 180 190 200 gvwsavvpgd werarytyia cinrvwreav dpyatavsvn gefgvvidwe 210 220 230 240 250 ktklappslp lpplcsptda iiyelsirdf tshpdsgavh kgkylglaet 260 270 280 …

    Get Price
  • Downsizing a pullulanase to a small molecule with

    2021-4-5 · The pullulanase is widely used in the baking and cyclodextrin production industry. If the stability of the enzyme is improved, then it becomes useful in several industrial applications.

    Get Price
  • Pullulanase M2 Bacillus licheniformis Enzyme |

    One Unit of pullulanase M2 activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40 o C.

    Get Price
  • Improvement of the Thermostability and Activity of ...

    Pullulanase is a commonly used starch-debranching enzyme with broad application in food, chemical and pharmaceutical industries. Since the starch-debranching process requires a high temperature, a thermostable pullulanase is desirable. In this study, based on the strategy of surficial residue replac …

    Get Price
  • Downsizing a pullulanase to a small molecule with

    2016-1-14 · Pullulanase is a debranching enzyme that can degrade the α-1,6-linkages of pullulan, amylopectin, and other branched polysaccharides [].In the grain industry, pullulanase is often used with glucoamylase or β-amylase to produce high glucose and maltose syrups [2, 3].In addition to increasing the sugar yield, pullulanase reduces reaction time, allows for high substrate concentrations, and ...

    Get Price
  • Production of a Thermostable Pullulanase in Bacillus ...

    2020-4-10 · Pullulanase is a debranching enzyme commonly used in the starch processing industry. Production of a thermostable pullulanase with high activity is desired due to the application of the enzyme to starch processing at high temperature. In this study, a thermostable pullulanase with high activity is overproduced in Bacillus subtilis.

    Get Price
  • Safety evaluation of the food enzyme pullulanase from a ...

    The food enzyme pullulanase (pullulan 6-a-glucanohydrolase; EC 3.2.1.41) is produced with a genetically modified Bacillus licheniformis (strain DP-Dzp39) by Danisco US Inc. The genetic modifications do not give rise to safety concerns. The food enzyme is free from viable cells of the production organism and its recombinant DNA.

    Get Price
  • Frontiers | Enhanced Expression of Pullulanase in

    2018-11-2 · Pullulanase plays an important role as a starch hydrolysis enzyme in the production of bio-fuels and animal feed, and in the food industry. Compared to the methods currently used for pullulanase production, synthesis by Bacillus subtilis would be safer and easier. However, the current yield of pullulanase from B. subtilis is low to meet industrial requirements.

    Get Price
  • Pullulanase - Wikipedia

    2021-4-14 · Pullulanase (EC 3.2.1.41, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, alpha-dextrin endo-1,6-alpha-glucosidase, R-enzyme, pullulan alpha-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram …

    Get Price
  • Preparation and characterization of pullulanase

    2015-10-28 · Pullulanase enzymatic hydrolyzate for DBS samples with higher degrees of debranching inclined towards retrogradation at 20 °C. DBSs with higher degrees of debranching could form a hydrogel with higher G′ and G′′ values, indicating these samples formed a stronger gel network. L-DBS could hold more water and its digestibility was higher.

    Get Price
  • Pululanase - Wikipedia bahasa Indonesia,

    2021-5-3 · Pululunase adalah enzim pemecah ikatan α-1,6 pada gugus makromolekul karbohidrat, seperti pati.[1] Ikatan α-1,6 berperan dalam pembentukan struktur percabangan pada karbohidrat.[1] Bersama dengan enzim alfa-amilase, pululunase dapat menghasilkan pemotongan molekul karbohidrat yang sempurna.[2] Enzim ini dapat diperoleh pada ekstrak beras dan ...

    Get Price
  • Pullulanase — Wikipédia

    2021-5-12 · La pullulanase est une glycoside hydrolase qui catalyse l'hydrolyse des liaisons osidiques α-D-(16) du pullulane, de l'amylopectine et du glycogène, ainsi que celles des dextrines limites α et β de ces deux derniers. Les pullulanases de type I attaquent spécifiquement les liaisons α-(16) tandis que les pullulanases de type II sont également capables d'hydrolyser les liaisons α-(1 ...

    Get Price
  • Production of a Thermostable Pullulanase in Bacillus ...

    2020-4-10 · Pullulanase is a debranching enzyme commonly used in the starch processing industry. Production of a thermostable pullulanase with high activity is desired due to the application of the enzyme to starch processing at high temperature. In this study, a thermostable pullulanase with high activity is overproduced in Bacillus subtilis.

    Get Price
  • Pullulanase - Wikipedia

    2021-4-14 · Pullulanase (EC 3.2.1.41, limit dextrinase, amylopectin 6-glucanohydrolase, bacterial debranching enzyme, debranching enzyme, alpha-dextrin endo-1,6-alpha-glucosidase, R-enzyme, pullulan alpha-1,6-glucanohydrolase) is a specific kind of glucanase, an amylolytic exoenzyme, that degrades pullulan. It is produced as an extracellular, cell surface-anchored lipoprotein by Gram …

    Get Price
  • Pullulanase Variants and Uses Thereof - NOVOZYMES

    2013-1-17 · Pullulanase Activity: Pullulanase activity means the ability to hydrolyze glycosidic alpha-(1,6)-linkages. It may be determined with pullulan or amylopectin as substrate, e.g., by the NPUN assay described below in the “Materials & Methods”-section or the AZCL-pullulan plates assay described in …

    Get Price
  • Safety evaluation of the food enzyme pullulanase from a ...

    The food enzyme pullulanase (pullulan 6-a-glucanohydrolase; EC 3.2.1.41) is produced with a genetically modified Bacillus licheniformis (strain DP-Dzp39) by Danisco US Inc. The genetic modifications do not give rise to safety concerns. The food enzyme is free from viable cells of the production organism and its recombinant DNA.

    Get Price
  • Mutational Analysis of the Pullulanase-Type

    Plants contain two types of α(16) glucan hydrolase (starch-debranching enzyme [DBE]). Mutations that affect the pullulanase-type DBE have not been described, although defects in isoamylase-type DBE, known in many plant species, indicate a function in starch biosynthesis. We describe a null mutation of a pullulanase-type DBE gene, a Mutator insertion in maize Zpu1 .

    Get Price
  • Pullulanase — Wikipédia

    2021-5-12 · La pullulanase est une glycoside hydrolase qui catalyse l'hydrolyse des liaisons osidiques α-D-(16) du pullulane, de l'amylopectine et du glycogène, ainsi que celles des dextrines limites α et β de ces deux derniers. Les pullulanases de type I attaquent spécifiquement les liaisons α-(16) tandis que les pullulanases de type II sont également capables d'hydrolyser les liaisons α-(1 ...

    Get Price
  • Gene cloning and enzymatic characterization of alkali ...

    2020-5-7 · Key words: pullulanase, debranching, GSP 中图分类号: TQ35 引用本文 朱昌玲, 雷鹏, 张峰伦, 张焕仕. 皂荚多糖的普鲁兰酶脱分支改性研究[J]. 生物质化学工程, 2021, 55(3): 42-46. Changling ZHU, Peng LEI, Fenglun ZHANG, Huanshi ZHANG. Debranching ...

    Get Price
  • Structural basis of carbohydrate binding in domain C

    2014-10-1 · Pullulanase activity was determined by measuring the amount of reducing sugars released during incubation of pullulanase with pullulan. 5 ml enzyme solution was added to 5 ml of 0·5% (wt/vol) pullulan dissolved in a 50 mmol l −1 citric acid‒Na 2 HPO 4 buffer (pH 6·0), and the samples were incubated at 50°C for 10 min.

    Get Price
  • Pullulanase: Role in Starch Hydrolysis and Potential ...

    2021-3-26 · Pullulanase capable of hydrolysing a-1,6 linkages of polymer is widely used in saccharification process for production of various useful materials such as maltose, amylose, and glucose, by debranching starch with and without a-amylase, ^-amylase, or glucoamylase, respectively. Clearly, pullulanase could facilitate a major change in the current ...

    Get Price
  • ENZYME - 3.2.1.41 Pullulanase

    Pullulanase. Alternative Name(s) Alpha-dextrin endo-1,6-alpha-glucosidase. Amylopectin 6-glucanohydrolase. Debranching enzyme. Limit dextrinase. Pullulan 6-glucanohydrolase. Reaction catalysed; Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and ...

    Get Price
  • PULLULANASE FROM BACILLUS DERAMIFICANS

    2018-7-14 · PULLULANASE FROM BACILLUS DERAMIFICANS EXPRESSED IN BACILLUS LICHENIFORMIS thNew specifications prepared at the 74 JECFA (2011) and published in FAO JECFA Monographs 11 (2011). An ADI 'not specified' was established at the 74 th JECFA (2011). SYNONYMS Pullulan α-1,6-glucanohydrolase; amylopectin 6-glucanohydrolase, bacterial debranching enzyme; …

    Get Price
  • Group B Streptococcus Pullulanase Crystal Structures

    The group B streptococcus type I pullulanase (SAP) is a class 13 glycoside hydrolase that is anchored to the bacterial cell surface via a conserved C-terminal anchoring motif and involved in α-glucan degradation. Recent in vitro functional studies have shown that SAP is immunogenic in humans and that anti-SAP sera derived from immunized animals impair both group A and group B streptococcus ...

    Get Price
  • The N‐Terminal Domain of the Pullulanase from ...

    2014-1-7 · The optimum temperature of the pullulanase was between 55 and 60 oC with a half-life as long as 48 h at 60 oC; and its optimum pH was between 5.6 and 6.4. Vmax and Km of the pullulanase was measured as 750 U/mg and 1.47 mg/mL, which is the highest

    Get Price
  • Preparation and characterization of pullulanase

    2018-3-1 · Anoxybacillus sp. WB42 pullulanase (PulWB42) is a novel thermophilic amylopullulanase that was assigned to the glycoside hydrolase family 13 subfamily 14 (GH13_14) type I pullulanases in the carbohydrate-active enzymes database. Its N-terminal domain (Met1–Phe101) was identified as the carbohydrate-binding module 68 (CBM68) by homology modeling.

    Get Price
  • Enhancement of pullulanase production from

    2015-10-28 · Pullulanase enzymatic hydrolyzate for DBS samples with higher degrees of debranching inclined towards retrogradation at 20 °C. DBSs with higher degrees of debranching could form a hydrogel with higher G′ and G′′ values, indicating these samples formed a stronger gel network. L-DBS could hold more water and its digestibility was higher.

    Get Price
Learn More Information About Our Products Know More